• Seeing is believing - we make iT happen

Cancer cell biology:

Feridani IA, Holmqvist B, H-O Sjogren, Strand S-E, Tennvall J, Baldetorp B (2007) Combined flow cytometry and confocal laser scanning microscopy for evaluation of BR96 antibody cancer cell targeting and internalization. J Cytometry A71A:361-370

Mossberg A, Hou Y, Svensson M, Holmqvist B, Svanborg C (2010) HAMLET treatment delays bladder cancer. J Urology 183:1590-1597

Persson O, Salford LG, Fransson J, Widegren B, Borrebaeck CAK, Holmqvist B (2010) Distribution, cellular localization and therapeutic potential of the tumor associated antigen Ku70/80 in glioblastoma multiforme. J Neurooncology 97:207-215


In vivo microscopy:

Yamatodani T, Holmqvist B, Kjéllen E, Oh P, Johnsson A, Mineta H, Wennerberg J (2011) Bevacizumab (Avastin) effects on xenografted human squamous carcinoma tumors – An intravital microscopical study in a mouse dorsal skinfold model. (Submitted).


Molecular cloning and in situ hybridization

Forsell J, Holmqvist B, Ekström P (2002) Molecular identification and developmental expression of UV and green opsin mRNAs in the pineal organ of the Atlantic halibut. Dev Brain Res 136:51-62

Holmqvist B, Ellingsen B, Alm P, Forsell J, Øyan A, Goksøyr A, Fjose A, Seo HC (2000) Identification and distribution of nitric oxide synthase in the brain of adult zebrafish. Neurosci Lett 292:119-122

Øyan A, Goksør A, Holmqvist B (2000) Partial cloning of neuronal nitric oxide synthase and expression in the brain of adult Atlantic salmon. Mol Brain Res 78:38-49



Nässel DR, Ekström P (1996) Neuropeptide immunocytochemistry. In Methods Mol Biol Vol. 72: Neurotransmitter methods. Rayne RC (ed.), Humana Press, Totowa NJ, pp 71-101

Parkefelt L, Skogh A-C, Nilsson D-E, Ekström P (2005) Bilateral symmetric organization of neural elements in the visual system of a coelenterate, Tripedalia cystophora (Cubozoa). J Comp Neurol 492:251-262


Neural tract tracing and multiple immunofluorescence labelling

Ekström P (1985) Anterograde and retrograde filling of central neural systems with horseradish peroxidase under in vitro conditions. J Neurosci Methods 15:21-35

Holmqvist BI, Östholm T, Ekström P (1992) DiI tracing in combination with immunocytochemistry for analysis of connectivities and chemoarchitectonics of specific neural systems in a teleost, the Atlantic salmon. J Neurosci Meth 42:45-63

Nylén A, Skagerberg G, Alm P, Larsson B, Holmqvist B, Andersson K-E (2001) Nitric oxide synthase in the hypothalamic paraventricular nuclei of the female rat; organization of spinal projections and coexistence with oxytocin or vasopressin. Brain Res 908:10-24


Receptor autoradiography

Ekström P, Vanecek J (1992) Localization of 2-[125I]-melatonin binding sites in the brain of the salmon Salmo salar L. Neuroendocrinology 55:529-537

Holmqvist BI, Carlberg M (1992) Galanin receptors in the brain of a teleost; Autoradiographic distribution of binding sites in the Atlantic salmon. (Salmo salar). J Comp Neurol 326:44- 60


Confocal microscopy

Ekström P. (2000) Confocal microscopy. Curr Protocols Toxicol, Vol. 1. Maines MD, Costa LG, Reed DJ, Sassa S, Sipes IG (eds). John Wiley & Sons, New York. 2.8.1-21

Ekström P, Garm A, Pålsson J, Vihtelic TS, Nilsson DE (2008) Immunohistochemical evidence for multiple photosystems in box jellyfish. Cell Tissue Res 333:115-124


Electron microscopy

Ekström P (1987) Photoreceptors and CSF-contacting neurons in the pineal organ of a teleost fish have direct axonal connections with the brain: an HRP-electron microscopic study. J Neurosci 7:987-995

Ekström P, Meissl H (1990) Electron microscopic analysis of S-antigen- and serotonin-immunoreactive neural and sensory elements in the photosensory pineal organ of the salmon. J Comp Neurol 292:73-82

Holmqvist B, Ekström P (1997) Subcellular localization of neuronal nitric oxide synthase immunoreactivity in the brain of a teleost, the Atlantic salmon. Brain Res 745:67-82

Nässel DRN, Holmqvist BI, Movérus BJA (1989) Vasopressin- and proctolin-like immunoreactive efferent neurons in the blowfly abdominal ganglia: development and ultrastructure. J Comp Neurol 283:450-463