Methods
Methods
Molecular cloning and in situ hybridization
Forsell J, Holmqvist B, Ekström P (2002) Molecular identification and developmental expression of UV and green opsin mRNAs in the pineal organ of the Atlantic halibut. Dev Brain Res 136:51-62
Holmqvist B., Ellingsen, B., Alm P, Forsell J, Øyan A, Goksøyr A, Fjose A, Seo HC (2000) Identification and distribution of nitric oxide synthase in the brain of adult zebrafish. Neurosci Lett 292:119-122
Øyan A, Goksøyr A, Holmqvist B (2000) Partial cloning of neuronal nitric oxide synthase and expression in the brain of adult Atlantic salmon. Mol Brain Res 78:38-49
Immunocytochemistry
Nässel DR, Ekström P (1996) Neuropeptide immunocytochemistry. In Methods Mol Biol Vol. 72: Neurotransmitter methods. Rayne RC (ed.), Humana Press, Totowa NJ, pp 71-101
Neural tract tracing and multiple immunofluorescence labelling
Ekström P (1985) Anterograde and retrograde filling of central neural systems with horseradish peroxidase under in vitro conditions. J Neurosci Methods 15:21-35
Holmqvist BI, Östholm T, Ekström P (1992) DiI tracing in combination with immunocytochemistry for analysis of connectivities and chemoarchitectonics of specific neural systems in a teleost, the Atlantic salmon. J Neurosci Meth 42:45-63
Nylén A, Skagerberg G, Alm P, Larsson B, Holmqvist B, Andersson K-E (2001). Nitric oxide synthase in the hypothalamic paraventricular nuclei of the female rat; organization of spinal projections and coexistence with oxytocin or vasopressin. Brain Res 908:10-24
Yanez J, Anadon R, Holmqvist BI, Ekström P (1993) Neural projections of the pineal organ in the larval sea lamprey (Petromyzon marinus, L.) revealed by indocarbocyanine dye tracing. Neurosci Lett 164:213-216
Receptor autoradiography
Ekström P, Vanecek J (1992) Localization of 2-[125I]-melatonin binding sites in the brain of the salmon Salmo salar L. Neuroendocrinology 55:529-537
Holmqvist BI, Carlberg M (1992) Galanin receptors in the brain of a teleost; Autoradiographic distribution of binding sites in the Atlantic salmon. (Salmo salar). J Comp Neurol 326:44- 60
Confocal microscopy
Ekström P (2000) Confocal microscopy. Curr Protocols Toxicol, Vol. 1. Maines MD, Costa LG, Reed DJ, Sassa S, Sipes IG (eds). John Wiley & Sons, New York. 2.8.1-21
Electron microscopy
Ekström P (1987) Photoreceptors and CSF-contacting neurons in the pineal organ of a teleost fish have direct axonal connections with the brain: an HRP-electron microscopic study. J Neurosci 7:987-995
Ekström P, Meissl H (1990) Electron microscopic analysis of S-antigen- and serotonin-immunoreactive neural and sensory elements in the photosensory pineal organ of the salmon. J Comp Neurol 292:73-82
Holmqvist B, Ekström P (1997) Subcellular localization of neuronal nitric oxide synthase immunoreactivity in the brain of a teleost, the Atlantic salmon. Brain Res 745:67-82
Nässel DRN, Holmqvist BI, Movérus BJA (1989) Vasopressin- and proctolin-like immunoreactive efferent neurons in the blowfly abdominal ganglia: development and ultrastructure. J Comp Neurol 283:450-463